Advances in sequencing of genomes have leaded to the promise of genetically modified fuel crop such Jatropha curcas that is used to obtain Jatropha oil. The gene regulation via Ribosome inhibiting Protein in the shrub has been studied using transfection of clones of curcin genes found in Jatropha curcas in other plants. This can lead to better cultivability of the Shrub and higher yield of Jatropha oil. NGS, Next-Generation Sequencing machines available commercially are tabulated. The triglycerides in Jatropha oil can be transesterified using excess methanol in the presence of catalyst or excess methanol into biodiesel and glycerol. The glycerol can be sold as by-product. The kinetics of consecutive-competitive reactions during methanolysis of triglycerides with intermediates such as diglyceride, monoglyceride are modeled. The model solutions assuming irreversible simple first order kinetics were obtained using the method of Laplace transforms. Although biodiesel forms in each consecutive step under some conditions of reaction rate ratios ω and κ the yield of glycerol are seen to be higher than that of biodiesel. This can be called “cross-over” and can be seen in the illustrations shown as prototypical examples. The operating cost of separating biodiesel from glycerol using centrifuge can be obtained from computer simulations of layer formation during shear flow. For optimal total cost there exists a yield of biodiesel where the process can be operated at.