Research Article
Volume 8 Issue 5 - 2021
Cardioprotective Potential of Beta vulgaris (Beetroot) in Doxorubicin-Induced Cardiac Injury among Male Albino Wistar Rats
Peter P Erigbali1*, Olubunmi O Ezomoh2 and Sule O Jimoh2
1Department of Human Physiology, Faculty of Basic Medical Sciences, Niger Delta University, Nigeria
2Department of Biochemistry, Faculty of Basic Medical Sciences, Niger Delta University, Nigeria
*Corresponding Author: Peter P Erigbali, Department of Human Physiology, Faculty of Basic Medical Sciences, Niger Delta University, Nigeria.
Received: March 22, 2021; Published: April 14, 2021




Abstract

We investigated the effect of pretreatment of adult male albino rats with Beta vulgaris (beetroot) in doxorubicin-induced cardiac injury experimental model. Twenty-four (24) animals after acclimatization for two (2) weeks, were randomly distributed into four (4) groups, each containing six (6) and exposed to treatments for twenty (20) days as follows: Group 1 (normal control) received normal saline, group 3 received 200 mg/kg of beetroot and group 4 received 400 mg/kg of beetroot orally (from day 1 to 20). All the rats in groups 2 (positive control) to 4 received 1.25 mg/kg bodyweight of doxorubicin intraperitoneally on day 15 of study. Animals were sacrificed on day 21 and blood sample was collected through cardiac puncture, into plain tubes for biochemical analysis, while the heart was surgically removed for histological analysis. The results showed that bodyweight was significantly (p < 0.05) increased in beetroot-pretreated rats; group 3 (211.75 ± 11.56) and group 4 (221.75 ± 2.21) when compared to normal control (194.00 ± 11.43) and positive control (201.55 ± 21.95) groups. There was significant (p < 0.05) increase in serum level of Lactate dehydrogenase, LDH (16.62 ± 3.55), Aspartate Transferase, AST (64.50 ± 8.02) and Alanine aminotransferase, ALT (56.67 ± 3.83) in the positive control group (2) indicating Doxorubicin-induced cardiac injury. However, in rats pretreated with beetroot, we observed significantly (p < 0.05) decreased serum levels of LDH in group 3 (12.65 ± 2.67) and group 4 (10.70 ± 2.85), AST in group 3 (45.67 ± 1.97) and group 4 (43.00 ± 2.37), as well as ALT in group 3 (43.67 ± 8.38) and group 4 (36.50 ± 6.92) when compared to positive control. Also, the difference in serum levels of these biochemical parameters between group 3 and 4 is significant (p < 0.05). Furthermore, histological analysis showed distorted myofibrils, loss of myocyte nuclei and orientation resulting in overall loss of myocardial architecture in positive control group, meanwhile appearance of marked improvement in the heart tissues of rats pretreated with beetroot was observed when compared to the positive and normal control. In this study, Beta vulgaris (beetroot) seems to have role in healthy weight gain in the rats and a cardio protective potential which benefit on the rats is in a manner that appear to be dose-dependent.

 

Keywords: Beetroot; Doxorubicin; Biochemical Parameters; Cardio Protective

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Citation: Peter P Erigbali., et al“Cardioprotective Potential of Beta vulgaris (Beetroot) in Doxorubicin-Induced Cardiac Injury among Male Albino Wistar Rats”. EC Cardiology 8.5 (2021): 11-18.

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