Research Article
Volume 20 Issue 8 - 2021
Efficacy of i-PRF as a Local Drug Delivery with Scaling and Root Planing in the treatment of Chronic Periodontitis
Hanku Ramavath1, Juliet Josephin2, Rajababu Palaparthy3, Mounika Basavaraju4, Abhinav Atchuta5 and Suryakanth Malgikar5*
1Senior Resident, Department of Periodontology, Government Dental College and Hospital, Hyderabad, Telangana, India
2Department of Periodontology, Reader, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India
3Professor, Department of Periodontology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India
4Senior Lecturer, Department of Periodontology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India
5Reader, Department of Periodontology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India
*Corresponding Author: Suryakanth Malgikar, Reader, Department of Periodontology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India.
Received: May 25, 2021; Published: July 29, 2021


Aim: The present study was planned to evaluate clinically the efficacy of i-Platelet Rich Fibrin injected locally into the periodontal pocket.

Materials and Methods: A total of 30 sites with pocket depths 5 - 6 mm were chosen as a split mouth design. Selected sites were randomly divided into two groups to receive scaling and root planing with injectable Platelet Rich Fibrin (SRP+i-PRF) (Test) and scaling and root planing (SRP) alone (Control). Clinical parameters like modified sulcular bleeding index (mSBI) and probing pocket depth (PPD) was recorded at baseline and 3 months interval.

Results: A significant reduction in mSBI, probing pocket depth (PPD), was observed from base line to 3 months in both the groups. However, results were found to be statistically significant in the SRP+i-PRF group (test).

Conclusion: SRP+i-PRF group showed positive results, however further studies are needed to evaluate i-PRF as a local drug delivery.

Clinical Significance: There is currently widespread interest for the use of various biomaterials to locally deliver biomolecules for regeneration. This article focuses on the use of liquid version of platelet rich fibrin as a local drug delivery for the fast delivery of growth factors.

Keywords: Chronic Periodontitis; i-PRF; Local Drug Delivery; Scaling and Root Planing


  1. Carranza FA., et al. “Carranza's Clinical Periodontology”. 10th Edition 494.
  2. Ximenez-Fyvie LA., et al. “Comparison of the microbiota of supra- and subgingival plaque in health and periodontitis”. Journal of Clinical Periodontology 9 (2000): 648-657.
  3. Newman MG., et al. “A 6-Month Multi-Center Evaluation of Adjunctive Tetracycline Fiber Therapy Used in Conjunction With Scaling and Root Planing in Maintenance Patients: Clinical Results”. Journal of Periodontology 7 (1994): 684-688.
  4. Sadaf N., et al. “Evaluation of efficacy of tetracycline fibers in conjunction with scaling and root planing in patients with chronic periodontitis”. Journal of Indian Society of Periodontology 3 (2012): 392-397.
  5. Chow TW., et al. “Importance of plasma fibronectin in determining PFP and PRP clot mechanical properties”. Thrombosis Research 2 (1983): 243-248.
  6. Delaini F., et al. “Enhanced affinity for arachidonic acid in platelet-rich plasma from rats with adriamycin induced nephrotic syndrome”. Thrombosis and Haemostasis 3 (1982): 260-262.
  7. Choukroun J., et al. “Une opportunite en paro-implantologie: le PRF”. Implantodontie55 (2001): 62.
  8. Miron R., et al. “Platelet Rich Fibrin and Soft Tissue Wound Healing: A Systematic Review”. Tissue Engineering Part B: Reviews 1 (2017): 83-99.
  9. Ali S., et al. “Platelet-Rich Fibrin in Maxillary Sinus Augmentation: a Systematic Review”. The Journal of Oral Implantology 6 (2015): 746-753.
  10. Lopez-Jornet P., et al. “Medication-related osteonecrosis of the jaw: Is autologous platelet concentrate application effective for prevention and treatment? A systematic review”. Journal of Cranio-Maxillofacial Surgery 8 (2016): 1067-1072.
  11. Malavolta EA., et al. “Comments on: Evaluation of platelet-rich plasma and fibrin matrix to assist in healing and repair of rotator cuff injuries: A systematic review and meta-analysis”. Clinical Rehabilitation7 (2016): 726-727.
  12. Moraschini V and Barboza Edos S. “Use of Platelet-Rich Fibrin Membrane in the Treatment of Gingival Recession: A Systematic Review and Meta-Analysis”. Journal of Periodontology 3 (2016): 281-290.
  13. Owen CA and Campbell EJ. “The cell biology of leukocyte-mediated proteolysis”. Journal of Leukocyte Biology 2 (1999): 137-150.
  14. Choukroun J and Ghanaati S. “Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients’ own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept”. European Journal of Trauma and Emergency Surgery1 (2018): 87-95.
  15. El Bagdadi K., et al. “Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept)”. The European Journal of Trauma and Emergency Surgery3 (2019): 467-479.
  16. Miron RJ., et al. “Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry?” Clinical Oral Investigations8 (2017): 2619-2627.
  17. Ghanaati S., et al. “Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells”. The Journal of Oral Implantology6 (2014): 679-689.
  18. Fujioka-Kobayashi M., et al. “Optimized platelet rich fibrin with the low speed concept: growth factor release, biocompatibility and cellular response”. Journal of Periodontology1 (2017): 112-121.
  19. Joshi CP., et al. “Autologous platelet rich fibrin as a potential antiperiopathogenic agent: An in-vitro study”. IP International Journal of Periodontology and Implantology2 (2016): 50-54.
  20. Miron RJ., et al. “Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry?” Clinical Oral Investigations8 (2017): 2619-2627.
  21. Listagarten MA., et al. “Effects of tetracycline and/or scaling on human periodontal disease, Clinical, microbiological and histopathological observation”. Journal of Clinical Periodontology4 (1978): 246-271.
  22. Lindhe J., et al. “Local tetracycline delivery using hollow fiber devices in periodontal therapy”. Journal of Clinical Periodontology3 (1979): 141-149.
  23. Rao N., et al. “Treating osteomyelitis: antibiotics and surgery”. Plastic and Reconstructive Surgery1 (2011): 177s-87s.
  24. Heitz-Mayfield LJ. “Systemic antibiotics in periodontal therapy”. Australian Dental Journal1 (2009): S96-101.
  25. Bidault P., et al. “Risk of bacterial resistance associated with systemic antibiotic therapy in periodontology”. Journal of the Canadian Dental Association 73 (2007): 721-725.
  26. Tonetti MS., et al. “A controlled multicenter study of adjunctive use of tetracycline periodontal fibers in mandibular class II furcations with persistent bleeding”. Journal of Clinical Periodontology 25 (1998): 728-376.
  27. Mombelli A., et al. “Treatment of peri-implantitis by local delivery of tetracycline. Clinical, microbiological and radiological results”. Clinical Oral Implants Research 12 (2001): 287-294.
  28. Rathbone CR., et al. “Effect of various concentrations of antibiotics on osteogenic cell viability and activity”. Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society 29 (2011): 1070-1074.
  29. Botelho MA., et al. “Nanotechnology in ligature induced periodontitis: protective effect of a doxycycline gel with nanoparticules”. Journal of Applied Oral Science: Revista FOB 18 (2010): 335-342.
  30. Cattabriga M., et al. “Tetracycline fiber used alone or with scaling and root planing in periodontal maintenance patients: Clinical results”. Quintessence International6 (1996): 395-400.
  31. Hanes PJ and Purvis JP. “Local anti-infective therapy: Pharmacological agents. A Systematic Review”. Annals of Periodontology 1 (2003): 79-98.
  32. Baker PJ., et al. “Tetracycline and its derivatives strongly bind to and are released from the tooth surface in active form”. Journal of Periodontology 10 (1983): 580-585.
  33. Miron RJ., et al. “Use of platelet-rich fibrin in regenerative dentistry: a systematic review”. Clinical Oral Investigations 6 (2017): 1913-1927.
  34. Choukroun J., et al. “Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing”. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology3 (2006): 56-60.
  35. Dohan DM., et al. “Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution”. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology3 (2006): 37-44.
  36. Ghanaati S., et al. “Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells”. The Journal of Oral Implantology6 (2014): 679-689.
  37. Bielecki T., et al. “The role of leukocytes from L-PRP/L-PRF in wound healing and immune defense: new perspectives”. Current Pharmaceutical Biotechnology7 (2012): 1153-1162.
  38. Martin P. “Wound healing–Aiming for perfect skin regeneration”. Science5309 (1997): 75-81.
  39. Barrick B., et al. “Leukocyte proteinases in wound healing: roles in physiologic and pathologic processes”. Wound Repair and Regeneration6 (1999): 410-422.
  40. Fujioka-Kobayashi M., et al. “Optimized platelet rich fibrin with the low speed concept: growth factor release, biocompatibility and cellular response”. The Journal of Periodontology1 (2017): 112-127.
  41. Kobayashi E., et al. “Comparative release of growth factors from PRP, PRF, and advanced-PRF”. Clinical Oral Investigations9 (2016): 2353-2360.
  42. Schar MO., et al. “Platelet-rich concentrates differentially release growth factors and induce cell migration in vitro”. Clinical Orthopaedics and Related Research5 (2015): 1635-1643.
  43. Martino MM., et al. “Heparin-binding domain of fibrinogen binds growth factors and promotes tissue repair when incorporated within a synthetic matrix”. Proceedings of the National Academy of Sciences of the United States of America 12 (2013): 4563-4568.
  44. Badade PS., et al. “Antimicrobial effect of platelet-rich plasma and platelet-rich fibrin”. Indian Journal of Dental Research 3 (2016): 300-304.
  45. Kour P., et al. “Comparative evaluation of antimicrobial efficacy of platelet-rich plasma, platelet-rich fibrin, and injectable platelet rich fibrin on the standard strains of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans”. Contemporary Clinical Dentistry 2 (2018): S325-330.
  46. Badade PS., et al. “Antimicrobial effect of platelet‑rich plasma and platelet‑rich fibrin”. Indian Journal of Dental Research 3 (2016): 300‑304.
  47. Tang YQ., et al. “Antimicrobial peptides from human platelets”. Infection and Immunity 12 (2002): 6524‑6533.
  48. Anitua E. “Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants”. The International Journal of Oral and Maxillofacial Implants 4 (1999): 529-535.
  49. Anitua E., et al. “Implementation of a more physiological plasma rich in growth factor (PRGF) protocol: anticoagulant removal and reduction in activator concentration”. Platelets 5 (2016): 459-466.
Citation: Suryakanth Malgikar., et al. “Efficacy of i-PRF as a Local Drug Delivery with Scaling and Root Planing in the treatment of Chronic Periodontitis”. EC Dental Science 20.8 (2021): 39-50.

PubMed Indexed Article

EC Pharmacology and Toxicology
LC-UV-MS and MS/MS Characterize Glutathione Reactivity with Different Isomers (2,2' and 2,4' vs. 4,4') of Methylene Diphenyl-Diisocyanate.

PMID: 31143884 [PubMed]

PMCID: PMC6536005

EC Pharmacology and Toxicology
Alzheimer's Pathogenesis, Metal-Mediated Redox Stress, and Potential Nanotheranostics.

PMID: 31565701 [PubMed]

PMCID: PMC6764777

EC Neurology
Differences in Rate of Cognitive Decline and Caregiver Burden between Alzheimer's Disease and Vascular Dementia: a Retrospective Study.

PMID: 27747317 [PubMed]

PMCID: PMC5065347

EC Pharmacology and Toxicology
Will Blockchain Technology Transform Healthcare and Biomedical Sciences?

PMID: 31460519 [PubMed]

PMCID: PMC6711478

EC Pharmacology and Toxicology
Is it a Prime Time for AI-powered Virtual Drug Screening?

PMID: 30215059 [PubMed]

PMCID: PMC6133253

EC Psychology and Psychiatry
Analysis of Evidence for the Combination of Pro-dopamine Regulator (KB220PAM) and Naltrexone to Prevent Opioid Use Disorder Relapse.

PMID: 30417173 [PubMed]

PMCID: PMC6226033

EC Anaesthesia
Arrest Under Anesthesia - What was the Culprit? A Case Report.

PMID: 30264037 [PubMed]

PMCID: PMC6155992

EC Orthopaedics
Distraction Implantation. A New Technique in Total Joint Arthroplasty and Direct Skeletal Attachment.

PMID: 30198026 [PubMed]

PMCID: PMC6124505

EC Pulmonology and Respiratory Medicine
Prevalence and factors associated with self-reported chronic obstructive pulmonary disease among adults aged 40-79: the National Health and Nutrition Examination Survey (NHANES) 2007-2012.

PMID: 30294723 [PubMed]

PMCID: PMC6169793

EC Dental Science
Important Dental Fiber-Reinforced Composite Molding Compound Breakthroughs

PMID: 29285526 [PubMed]

PMCID: PMC5743211

EC Microbiology
Prevalence of Intestinal Parasites Among HIV Infected and HIV Uninfected Patients Treated at the 1o De Maio Health Centre in Maputo, Mozambique

PMID: 29911204 [PubMed]

PMCID: PMC5999047

EC Microbiology
Macrophages and the Viral Dissemination Super Highway

PMID: 26949751 [PubMed]

PMCID: PMC4774560

EC Microbiology
The Microbiome, Antibiotics, and Health of the Pediatric Population.

PMID: 27390782 [PubMed]

PMCID: PMC4933318

EC Microbiology
Reactive Oxygen Species in HIV Infection

PMID: 28580453 [PubMed]

PMCID: PMC5450819

EC Microbiology
A Review of the CD4 T Cell Contribution to Lung Infection, Inflammation and Repair with a Focus on Wheeze and Asthma in the Pediatric Population

PMID: 26280024 [PubMed]

PMCID: PMC4533840

EC Neurology
Identifying Key Symptoms Differentiating Myalgic Encephalomyelitis and Chronic Fatigue Syndrome from Multiple Sclerosis

PMID: 28066845 [PubMed]

PMCID: PMC5214344

EC Pharmacology and Toxicology
Paradigm Shift is the Normal State of Pharmacology

PMID: 28936490 [PubMed]

PMCID: PMC5604476

EC Neurology
Examining those Meeting IOM Criteria Versus IOM Plus Fibromyalgia

PMID: 28713879 [PubMed]

PMCID: PMC5510658

EC Neurology
Unilateral Frontosphenoid Craniosynostosis: Case Report and a Review of the Literature

PMID: 28133641 [PubMed]

PMCID: PMC5267489

EC Ophthalmology
OCT-Angiography for Non-Invasive Monitoring of Neuronal and Vascular Structure in Mouse Retina: Implication for Characterization of Retinal Neurovascular Coupling

PMID: 29333536 [PubMed]

PMCID: PMC5766278

EC Neurology
Longer Duration of Downslope Treadmill Walking Induces Depression of H-Reflexes Measured during Standing and Walking.

PMID: 31032493 [PubMed]

PMCID: PMC6483108

EC Microbiology
Onchocerciasis in Mozambique: An Unknown Condition for Health Professionals.

PMID: 30957099 [PubMed]

PMCID: PMC6448571

EC Nutrition
Food Insecurity among Households with and without Podoconiosis in East and West Gojjam, Ethiopia.

PMID: 30101228 [PubMed]

PMCID: PMC6086333

EC Ophthalmology
REVIEW. +2 to +3 D. Reading Glasses to Prevent Myopia.

PMID: 31080964 [PubMed]

PMCID: PMC6508883

EC Gynaecology
Biomechanical Mapping of the Female Pelvic Floor: Uterine Prolapse Versus Normal Conditions.

PMID: 31093608 [PubMed]

PMCID: PMC6513001

EC Dental Science
Fiber-Reinforced Composites: A Breakthrough in Practical Clinical Applications with Advanced Wear Resistance for Dental Materials.

PMID: 31552397 [PubMed]

PMCID: PMC6758937

EC Microbiology
Neurocysticercosis in Child Bearing Women: An Overlooked Condition in Mozambique and a Potentially Missed Diagnosis in Women Presenting with Eclampsia.

PMID: 31681909 [PubMed]

PMCID: PMC6824723

EC Microbiology
Molecular Detection of Leptospira spp. in Rodents Trapped in the Mozambique Island City, Nampula Province, Mozambique.

PMID: 31681910 [PubMed]

PMCID: PMC6824726

EC Neurology
Endoplasmic Reticulum-Mitochondrial Cross-Talk in Neurodegenerative and Eye Diseases.

PMID: 31528859 [PubMed]

PMCID: PMC6746603

EC Psychology and Psychiatry
Can Chronic Consumption of Caffeine by Increasing D2/D3 Receptors Offer Benefit to Carriers of the DRD2 A1 Allele in Cocaine Abuse?

PMID: 31276119 [PubMed]

PMCID: PMC6604646

EC Anaesthesia
Real Time Locating Systems and sustainability of Perioperative Efficiency of Anesthesiologists.

PMID: 31406965 [PubMed]

PMCID: PMC6690616

EC Pharmacology and Toxicology
A Pilot STEM Curriculum Designed to Teach High School Students Concepts in Biochemical Engineering and Pharmacology.

PMID: 31517314 [PubMed]

PMCID: PMC6741290

EC Pharmacology and Toxicology
Toxic Mechanisms Underlying Motor Activity Changes Induced by a Mixture of Lead, Arsenic and Manganese.

PMID: 31633124 [PubMed]

PMCID: PMC6800226

EC Neurology
Research Volunteers' Attitudes Toward Chronic Fatigue Syndrome and Myalgic Encephalomyelitis.

PMID: 29662969 [PubMed]

PMCID: PMC5898812

EC Pharmacology and Toxicology
Hyperbaric Oxygen Therapy for Alzheimer's Disease.

PMID: 30215058 [PubMed]

PMCID: PMC6133268

News and Events

February Issue Release

We always feel pleasure to share our updates with you all. Here, notifying you that we have successfully released the February issue of respective journals and the latest articles can be viewed on the current issue pages.

Submission Deadline for Upcoming Issue

ECronicon delightfully welcomes all the authors around the globe for effective collaboration with an article submission for the upcoming issue of respective journals. Submissions are accepted on/before February 17, 2023.

Certificate of Publication

ECronicon honors with a "Publication Certificate" to the corresponding author by including the names of co-authors as a token of appreciation for publishing the work with our respective journals.

Best Article of the Issue

Editors of respective journals will always be very much interested in electing one Best Article after each issue release. The authors of the selected article will be honored with a "Best Article of the Issue" certificate.

Certifying for Review

ECronicon certifies the Editors for their first review done towards the assigned article of the respective journals.

Latest Articles

The latest articles will be updated immediately on the articles in press page of the respective journals.