Research Article
Volume 20 Issue 9 - 2021
An In-Vitro Study on the Dimensional Stability of Self-Disinfecting Alginate Materials Using Different Concentrations of Chlorhexidine Solutions
Danish Muzaffar1, Abdur Rehman2*, Leena Binti Adam Muralidharan3, Leng Wei3, Ling Kwei Wen3, Lishaliniy a/p Lenga Gururuloo3, Priyank Seth4 and Rafey Ahmed Jameel5
1Senior Lecturer, Faculty of Dentistry, SEGi University, Kota Damansara, Selangor, Malaysia
2Associate Professor, Science of Dental Material, Hamdard University Dental Hospital, Hamdard University, Karachi, Pakistan
3Dental Officer, Ministry of Health, Malaysia
4Senior Lecturer, Faculty of Dentistry, SEGi University, Kota Damansara, Selangor, Malaysia
5Assistant Professor, Department of Oral Biology and Tooth Morphology, Dow University of Health Sciences, Karachi, Pakistan
*Corresponding Author: Abdur Rehman, Associate Professor, Science of Dental Material, Hamdard University Dental Hospital, Hamdard University, Karachi, Pakistan.
Received: July 14, 2021; Published: August 12, 2021


The aim of this study was to compare the dimensional changes in casts poured from irreversible hydrocolloid impression materials internally disinfected with different concentrations of chlorhexidine. In this in vitro study, type IV dental stone casts (Elite Stone, Zhermack) were fabricated from irreversible hydrocolloid impressions of Frasaco typodont dentulous model. The irreversible hydrocolloid powder (Kromopan, Lascod Italy) was mixed with four different concentrations of chlorhexidine solution at 0.05%, 0.10%, 0.15% and 0.2%. Distilled water was used as control. Twenty casts were made for each mixing solution. Four measurements including cross arch width (AB and CD) and sagittal plane distance (BC and DA) were measured with a Mitutoyo digital caliper with accuracy up to 0.01 mm. Data was analyzed using SPSS version 22. ANOVA and Post Hoc Tukey’s test were used to compare the mean differences among the groups. The mean cast measurements showed no significant difference in any of the three out of four measurements among the groups with different concentrations of chlorhexidine solutions, with p values being 0.716, 0.64 and 0.35 respectively. However, the DA measurement was significant with p = 0.002. Different concentrations of chlorhexidine solutions used to internally disinfect hydrocolloid impression materials have no significant impact on the dimensional stability of the casts.

Keywords: Irreversible Hydrocolloid Impressions; Dental Stone Casts and Chlorhexidine Solution


  1. Cervino Gabriele., et al. “Alginate Materials and Dental Impression Technique: A Current State of the Art and Application to Dental Practice”. Marine Drugs 171 (2019): 18.
  2. Choudhury Gopal Krishna., et al. “Disinfectant Efficacy of 0.525% Sodium Hypochlorite and Epimax on Alginate Impression Material”. The Journal of Contemporary Dental Practice1 (2018): 113-116.
  3. Al Mortadi Noor., et al. “Disinfection of Dental Impressions: Knowledge and Practice among Dental Technicians”. Clinical, Cosmetic and Investigational Dentistry 11 (2019): 103.
  4. Gupta Sakshi., et al. “Infection Control Knowledge and Practice: A Cross-Sectional Survey on Dental Laboratories in Dental Institutes of North India”. The Journal of the Indian Prosthodontic Society 17 4 (2017): 348.
  5. Sofou A., et al. “Contamination Level of Alginate Impressions Arriving at a Dental Laboratory”. Clinical Oral Investigations3 (2002): 161-65.
  6. Kugel Gerard., et al. “Disinfection and Communication Practices: A Survey: Of Us Dental Laboratories”. The Journal of the American Dental Association6 (2000): 786-792.
  7. No ADA Specification. "For Alginate Impression Material”. Revised ANSI/ADA Specification 18 (1992): 18.
  8. Khan Muhammad Waseem Ullah. "An Overview of Dental Impression Disinfection Techniques-a Literature Review”. Java Platform Debugger Architecture04 (2018): 208.
  9. Chidambaranathan Ahila S and Muthukumar Balasubramanium. "Comprehensive Review and Comparison of the Disinfection Techniques Currently Available in the Literature”. Journal of Prosthodontics 2 (2019): e849-e856.
  10. Poulos JG and LR Antonoff. "Disinfection of Impressions. Methods and Effects on Accuracy”. The New York State Dental Journal6 (1997): 34-36.
  11. Ginjupalli Kishore., et al. “Antimicrobial Activity and Properties of Irreversible Hydrocolloid Impression Materials Incorporated with Silver Nanoparticles”. The Journal of Prosthetic Dentistry6 (2016): 722-728.
  12. Ismail Hussien Abdalfatah., et al. “A Self-Disinfecting Irreversible Hydrocolloid Impression Material Mixed with Povidone Iodine Powder”. European Journal of Dentistry04 (2016): 507-511.
  13. Wang Jian., et al. “A Self-Disinfecting Irreversible Hydrocolloid Impression Material Mixed with Chlorhexidine Solution”. The Angle Orthodontist5 (2007): 894-900.
  14. Wandrekar Siddharth., et al. “Dimensional Stability of Newer Alginate Impression Materials over Seven Days”. The European Journal of Prosthodontics and Restorative Dentistry4 (2010): 163-70.
  15. Gümüş Hasan Ö., et al. “The Effect of Pouring Time on the Dimensional Stability of Casts Made from Conventional and Extended-Pour Irreversible Hydrocolloids by 3d Modelling”. Journal of Dental Sciences3 (2015): 275-281.
  16. Phyo Thida and Myat Nyan. "Effect of Mixing Methods and Disinfection on Dimensional Accuracy of Alginate Impression”. Myanmar Dental (2015): 21.
  17. Rehman, Abdur. "Comparison of Surface Detail Reproduction of Alginate Impression Material and Type Iii Dental Stone”. EC Dental Science 19 (2020): 01-06.
  18. Amalan Arul., et al. “Evaluation of Properties of Irreversible Hydrocolloid Impression Materials Mixed with Disinfectant Liquids”. Dental Research Journal1 (2013): 65.
  19. Ramer Michael S., et al. “Accuracy of Irreversible Hydrocolloid Impression Material Mixed with Disinfectant Solutions”. Journal of Prosthodontics3 (1993): 156-158.
  20. Alcan Toros., et al. “The Relationship between Digital Model Accuracy and Time-Dependent Deformation of Alginate Impressions”. The Angle Orthodontist1 (2009): 30-36.
Citation: Abdur Rehman., et al. “An In-Vitro Study on the Dimensional Stability of Self-Disinfecting Alginate Materials Using Different Concentrations of Chlorhexidine Solutions”. EC Dental Science 20.9 (2021): 07-14.

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