Research Article
Volume 20 Issue 10 - 2021
Personalized Culture of Human Dental Pulp Stem Cells
Miroslav Tolar1* and Marie M Tolarova2
1Pacific Regenerative Dentistry Laboratory, Departments of Orthodontics and Biomedical Sciences, University of the Pacific, Arthur A Dugoni School of Dentistry, San Francisco, California, CA, USA
2Pacific Craniofacial Team and Cleft Prevention Program, Departments of Biomedical Sciences and Orthodontics, Arthur A. Dugoni School of Dentistry, University of the Pacific, San Francisco, California, USA
*Corresponding Author: Miroslav Tolar, Pacific Regenerative Dentistry Laboratory, Departments of Orthodontics and Biomedical Sciences, University of the Pacific, Arthur A Dugoni School of Dentistry, San Francisco, California, CA, USA.
Received: May 07, 2021; Published: September 24, 2021




Abstract

Introduction: We studied interactions of human dental pulp-derived stem cells (hDPSC), human platelet-rich plasma (PRP), human platelet-rich fibrin (PRF) and fibrin in vitro. These factors are known to participate in repair and healing of a wound and PRP and PRF have been used clinically. Optimized combinations of PRP, PRF and a low oxygen atmosphere in vitro enhance multiplication of hDPSC and are highly important for their successful clinical applications.

Methods: Our in vitro model involved hDPSC isolated from extracted premolars and grown in MSC cultivation medium. Human PRP, PRF, fibrin and serum were prepared from venous blood. The cultures were maintained in normal 21% oxygen or in a low (5%) oxygen atmosphere (Biospherix). Properties of hDPSC and their responses in culture were examined by flow cytometry, growth dynamics, and histochemical staining of cells. The study has a valid IRB approval Nr. 09-99.1.

Results: We show that PRP, PRF, fibrin itself and low oxygen atmosphere markedly increase multiplication rate of human hDPSC in culture and support their osteogenic differentiation potential.

Conclusion: The personalized (optimized in respect to a patient) cultivation conditions can be used for accelerated preparation of patient’s hDPSC suitable for implantation to facilitate growth of a new bone.

Keywords: Dental Pulp Stem Cells; Platelet Rich Plasma; Platelet Rich Fibrin; Regenerative Dentistry

References

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  3. Dohan-Ehrenfest DM., et al. “Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF)”. Trends in Biotechnology 27/3 (2009): 158-167.
  4. Kolf CM., et al. “Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation”. Arthritis Research and Therapy 9 (2007): 204.
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  7. Tolar J and Tolar M. “Reinventing mesenchymal stromal cells”. Cytotherapy 14/4 (2012): 388-390.
  8. Hibi H., et al. “Alveolar cleft osteoplasty using tissue-engineered osteogenic material”. International Journal of Oral and Maxillofacial Surgery 35 (2006): 551-555.
  9. Mendonca JJ and Juiz-Lopez P. “Regenerative facial reconstruction of terminal stage osteoradionecrosis and other advanced craniofacial diseases with adult cultured stem and progenitor cells”. Plastic and Reconstructive Surgery 126/5 (2010): 1699-1709.
  10. Centeno CJ., et al. “Safety and complications reporting update on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique”. Current Stem Cell Research and Therapy 6/4 (2011): 368-378.
Citation: Miroslav Tolar and Marie M Tolarova. “Personalized Culture of Human Dental Pulp Stem Cells”. EC Dental Science 20.10 (2021): 62-67.

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