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Research Article
Volume 6 Issue 4 - 2021
Evaluating the Effects of Life styles and History of Exposure to Radiation on Levels of Significance and Severity of Sperm DNA Damage among Males with Infertility Using 8-Hydroxydeoxyguanosine (8-OHDG) as a Marker
Oladosu Waliu Olatunbosun1*, Biliaminu Sikiru Abayomi2, Abdulazeez Ishola Musbau2, Alayo Adewale Mukaila3, Ahmed Amina Olayinka3 and Okesina Adekunle Bashiru2
1Department of Chemical Pathology, Federal Medical Centre, Abeokuta, Ogun State, Nigeria
2Department of Chemical Pathology, University of Ilorin, Kwara State, Nigeria
3Department of Obstetrics and Gynecology, Federal Medical Centre, Abeokuta, Ogun State, Nigeria
*Corresponding Author: Oladosu Waliu Olatunbosun, Department of Chemical Pathology, Federal Medical Centre, Abeokuta, Ogun State, Nigeria.
Received: March 14, 2021; Published: March 25, 2021


Background: Sperm DNA damage has been established as a known cause of male infertility and severe sperm DNA damage has been linked to failure of some assisted forms of reproductive techniques. While there are different sources of DNA damage, Reactive oxygen species (ROS) are the most clinically relevant sources and this can be quantified by seminal 8-OHDG assay.

This study evaluated the effects of lifestyles and exposure to radiation on sperm DNA damage.

Methods This was a descriptive cross sectional study of 120 males with at least one SFA parameter abnormalities as test subjects and 120 males with normal SFA results as controls. Seminal 8-OHDG was assayed as a marker of sperm DNA damage using ELISA method.  

Results Significant sperm DNA damage, taken as seminal 8-OHDG levels ≥20.45ng/ml was statistically more associated with smoking, P = 0.001 vs 0.913, Alcohol consumption, P= 0.001 vs 0.759, Exposure to radiation, including mobile phones, P = 0.05 vs 0.699,  among test subjects than controls respectively, but not associated with herbal concoction consumption, P=0.201 vs 0.456.  Severe sperm DNA damage, taken as seminal 8-OHDG ≥ 26.6ng/ml corresponding to DNA fragmentation index > 30%, was associated with alcohol consumption (P = 0.010), cigarette smoking (P = 0.001), STI (P = 0.027) while exposure to radiation especially from mobile phones was not associated with severe sperm DNA damage (P = 0.797) among test subjects.

Conclusion: Cigarette smoking, alcohol consumption, STI and keeping mobile phones in the pockets were associated with significant and all except histories of exposure to radiation were.


Keywords:  Sperm DNA Damage; Male Infertility; 8-Hydroxyl Deoxyguanosine; Lifestyle


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Citation: Oladosu Waliu Olatunbosun., et al. “Evaluating the Effects of Life styles and History of Exposure to Radiation on Levels of Significance and Severity of Sperm DNA Damage among Males with Infertility Using 8-Hydroxydeoxyguanosine (8-OHDG) as a Marker”. EC Gastroenterology and Digestive System 6.4 (2021): 15-27.

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