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Research Article
Volume 6 Issue 4 - 2021
Evaluating the Effects of Life styles and History of Exposure to Radiation on Levels of Significance and Severity of Sperm DNA Damage among Males with Infertility Using 8-Hydroxydeoxyguanosine (8-OHDG) as a Marker
Oladosu Waliu Olatunbosun1*, Biliaminu Sikiru Abayomi2, Abdulazeez Ishola Musbau2, Alayo Adewale Mukaila3, Ahmed Amina Olayinka3 and Okesina Adekunle Bashiru2
1Department of Chemical Pathology, Federal Medical Centre, Abeokuta, Ogun State, Nigeria
2Department of Chemical Pathology, University of Ilorin, Kwara State, Nigeria
3Department of Obstetrics and Gynecology, Federal Medical Centre, Abeokuta, Ogun State, Nigeria
*Corresponding Author: Oladosu Waliu Olatunbosun, Department of Chemical Pathology, Federal Medical Centre, Abeokuta, Ogun State, Nigeria.
Received: March 14, 2021; Published: March 25, 2021




Abstract

Background: Sperm DNA damage has been established as a known cause of male infertility and severe sperm DNA damage has been linked to failure of some assisted forms of reproductive techniques. While there are different sources of DNA damage, Reactive oxygen species (ROS) are the most clinically relevant sources and this can be quantified by seminal 8-OHDG assay.

This study evaluated the effects of lifestyles and exposure to radiation on sperm DNA damage.

Methods This was a descriptive cross sectional study of 120 males with at least one SFA parameter abnormalities as test subjects and 120 males with normal SFA results as controls. Seminal 8-OHDG was assayed as a marker of sperm DNA damage using ELISA method.  

Results Significant sperm DNA damage, taken as seminal 8-OHDG levels ≥20.45ng/ml was statistically more associated with smoking, P = 0.001 vs 0.913, Alcohol consumption, P= 0.001 vs 0.759, Exposure to radiation, including mobile phones, P = 0.05 vs 0.699,  among test subjects than controls respectively, but not associated with herbal concoction consumption, P=0.201 vs 0.456.  Severe sperm DNA damage, taken as seminal 8-OHDG ≥ 26.6ng/ml corresponding to DNA fragmentation index > 30%, was associated with alcohol consumption (P = 0.010), cigarette smoking (P = 0.001), STI (P = 0.027) while exposure to radiation especially from mobile phones was not associated with severe sperm DNA damage (P = 0.797) among test subjects.

Conclusion: Cigarette smoking, alcohol consumption, STI and keeping mobile phones in the pockets were associated with significant and all except histories of exposure to radiation were.

 

Keywords:  Sperm DNA Damage; Male Infertility; 8-Hydroxyl Deoxyguanosine; Lifestyle

References

  1. Agarwal A and Said TM. ‘‘Role of sperm chromatin abnormalities and DNA damage in male infertility’’. Human Reproduction Update 9 (2003): 331-345.
  2. Schulte RT., et al. ‘‘Sperm DNA damage in male infertility: etiologies, assays , and outcomes’’. Journal of Assisted Reproduction and Genetics 27 (2010): 3-12.
  3. Wright C. ‘‘Sperm DNA damage caused by oxidative stress : modifiable clinical , lifestyle and nutritional factors in male infertility’’. Reproductive Biomedicine Online 28 (2014): 684-703.
  4. Zabludovsky N., et al. ‘‘Relationship between human sperm lipid peroxidation, comprehensive quality parameters and IVF outcome’’. Andrologia 31 (1999): 91-98.
  5. Agarwal A and Allamaneni SSR. ‘‘Sperm DNA damage assessment : a test whose time has come’’. Middle East Fertility Society Journal 84 (2005)’’: 5-8.
  6. De kretser DM. ‘‘Male infertility’’. Lancet 349 (1997): 787-790.
  7. Sikka SC. ‘‘Oxidative stress and role of antioxidants in normal and abnormal sperm function’’. Frontiers in Bioscience 1 (1996): 78-86.
  8. Gharagozloo P and Aitken RJ. ‘‘The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy’’. Human Reproduction 26 (2011): 1628-1640.
  9. González-marín C. ‘‘Types , Causes , Detection and Repair of DNA Fragmentation in Animal and Human Sperm Cells’’. International Journal of Molecular Sciences (2012): 14026-14052.
  10. Belle G Van. ‘‘Biostatistics: A Methodology for the Health Sciences’’. Second (2004).
  11. Uhuonrenren OB., et al ‘‘The Prevalence and Patterns of Endocrinopathies Amongs Azoospermic Male Partners at a Fertility Clinic in Benin City’’. Endocrinology and Metabolism International Journal 1 (2014): 1-6.
  12. Chessbrough M. ‘‘District Laboratory Practise in Tropical Countries’’. (2006).
  13. Khaldoun S. ‘‘Sperm DNA fragmentation testing: To do or not to do?’’ Middle East Fertility Society Journal 18 (2013): 78-83.
  14. World Health Organization reference values for human semen Human Reproduction (2009).
  15. Wang Q., et al. ‘‘Increased Oxidative DNA Damage in Seminal Plasma of Infertile Men with Varicocele’’. Andrology 3 (2014): 1-6.
  16. Wayne P. ‘‘Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory; Approved Guideline’’. Clinical Laboratory Standard Institute (2008) C28-A3.
  17. Sharma R., et al. ‘‘Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay using bench top flow cytometer for evaluation of sperm DNA fragmentation in fertility laboratories: protocol, reference values, and quality control’’. Journal of Assisted Reproduction and Genetics 33 (2016): 291-300.
  18. Sharma R., et al. ‘‘TUNEL as a test for sperm DNA damage in the evaluation of male infertility’’. Urology 76 (2010): 1380-1386.
  19. Oleszczuk K., et al. ‘‘Prevalence of high DNA fragmentation index in male partners of unexplained infertile couples’’. Andrology 1(2013): 357-360.
  20. Arabi M and Moshtaghi H. ‘‘Influence of cigarette smoking on spermatozoa via seminal plasma’’. Andrologia 37 (2005): 119-124.
  21. Potts R. ‘‘Sperm chromatin damage associated with male smoking’’. Mutation Research 423 (1999): 103-111.
  22. Eftekhar M., et al. ‘‘Relation of Second Hand Smoker and Effect on Pregnancy Outcome and Newborns Parameters’’. Gynecology and Women’s Health Research 6 (2016): 2.
  23. Pembrey M., et al. ‘‘Sex-specific, maleline transgenerational responses in humans’’. European Journal of Human Genetics 14(2006): 159-166.
  24. Aboulmaouahib S., et al. ‘‘Impact of alcohol and cigarette smoking consumption in male fertility potential: Looks at lipid peroxidation, enzymatic antioxidant activities and sperm DNA damage’’. Andrologia 50 (2017): 3.
  25. Komiya A., et al. ‘‘Clinical Factors Associated with Sperm DNA Fragmentation in Male Patients with Infertility’’. The Scientific World Journal 2014 (2014): 868303.
  26. Wdowiak A., et al. ‘‘Relationship Between Alcohol Consumption and Sperm Nuclear DNA Fragmentation and Pregnancy’’. Advance Andrology Online 3 (2016): 14-21.
  27. Agarwal A., et al. ‘‘Role of oxidative stress, infection and inflammation in male infertility’’. Andrologia 11 (2018): 50.
  28. Gallegos G., et al. ‘‘Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma’’. Fertility and Sterility 2 (2008) 90: 328-334.
  29. Zalata A., et al. ‘‘In vitro effect of cell phone radiation on motility, DNA fragmentation and clusterin gene expression in human sperm’’. International Journal of Fertility and Sterility 9 (2015): 129-136.
Citation: Oladosu Waliu Olatunbosun., et al. “Evaluating the Effects of Life styles and History of Exposure to Radiation on Levels of Significance and Severity of Sperm DNA Damage among Males with Infertility Using 8-Hydroxydeoxyguanosine (8-OHDG) as a Marker”. EC Gastroenterology and Digestive System 6.4 (2021): 15-27.

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