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Research Article
Volume 5 Issue 1 - 2020
Laparoscopic Ovarian Drilling Regulates Endometrial LIF and Integrin Expression in PCOS
Ertan Zan1* and Bülent Duran2
1Private Clinic, Obstetrics and Gynecology, Alanya, Turkey
2Adatip Private Hospital, Obstetrics and Gynecology, IVF Centre, Sakarya, Turkey
*Corresponding Author: Ertan Zan, Private Clinic, Obstetrics and Gynecology, Alanya, Turkey.
Received: November 13, 2019; Published: December 12, 2019


The study was planned to investigate whether laparoscopic ovarian drilling (LOD) alters the expression levels of leukemia inhibitory factor (LIF) and integrin beta 3 (ITGB3) in the endometrium of infertile women with clomiphene-resistant PCOS. Expression of LIF and integrins mRNA in the endometrium obtained before and after LOD during the implantation window were measured. Expression of each gene was evaluated using real-time reverse transcriptase polymerase chain reaction (RT-PCR). Expression levels of endometrial LIF and integrin were lower in PCOS subjects before LOD compared to healthy fertile controls. Compared with fertile subjects LOD up-regulated endometrial LIF and integrin expression. Fold changes of LIF and integrin following LOD were found to be 3.12 and 3.88 respectively. Fold change increase in LIF and integrin were noted as statistically significant (P < .03, P < .01). LOD increses endometrial LIF and integrin expression and improves receptivity in clomiphene-resistant PCOS women.

Keywords: Laparoscopic Ovarian Drilling (LOD); Leukemia Inhibitory Factor (LIF); Integrin Beta 3 (ITGB3); Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)


  1. Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group. “Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome”. Fertility and Sterility 81.1 (2004): 19-25.
  2. Matthews DR., et al. “Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man”. Diabetologia 28.7 (1985): 412-419.
  3. El-Sayed MLM., et al. “Unilateral Versus Bilateral Laparoscopic Ovarian Drilling Using Thermal Dose Adjusted According to Ovarian Volume in CC-Resistant PCOS, A Randomized Study”. Journal of Obstetrics and Gynecology of India 67.5 (2017): 356-362. 
  4. Savaris RF., et al. “Progesterone resistance in PCOS endometrium: a microarray analysis in clomiphene citratetreated and artificial menstrual cycles”. Journal of Clinical Endocrinology and Metabolism 96.6 (2011): 1737-1746.
  5. Wang J., et al. “Evidence for a mullerian-inhibiting substance autocrine/paracrine system in adult human endometrium”. Fertility and Sterility 91.4 (2008): 1195-1203.
  6. Celik O., et al. “Laparoscopic endometrioma resection increases peri-implantation endometrial HOXA-10 and HOXA-11 mRNA expression”. Fertility and Sterility 104.2 (2015): 356-365.
  7. Celik O., et al. “Molecular role of peptides/proteins in subfertility of polycystic ovarian syndrome”. Cellular and Molecular Biology 65.3 (2019): 32-40. 
  8. Ibrahim MH., et al. “Letrozole versus laparoscopic ovarian drilling in infertile women with PCOS resistant to clomiphene citrate”. Middle East Fertility Society Journal 22.4 (2017): 251-254.
  9. Yu Q., et al. “Letrozole versus laparoscopicovarian drilling in clomiphene citrate-resistant women with polycystic ovary syndrome: a systematic review and meta-analysis of randomized controlled trials”. Reproductive Biology and Endocrinology 17.1 (2019): 17. 
  10. Elnashar A., et al. “Letrozole induction of ovulation in women with clomiphene citrate-resistant polycystic ovarysyndrome may not depend on the period of infertility, the body mass index, or the luteinizing hormone/follicle-stimulating hormone ratio”. Fertility and Sterility 85.2 (2006): 511-513.
  11. Abu Hashim H., et al. “Three decades after Gjönnaess’s laparoscopic ovarian drilling for treatment of PCOS whatdo we know? An evidence-based approach”. Archives of Gynecology and Obstetrics 288.2 (2013): 409-422.
  12. Bellver J., et al. “Endometrial gene expression in the window of implantation is altered in obese women especially in association with polycystic ovary syndrome”. Fertility and Sterility 95.7 (2011): 2335-2341.
  13. Cermik D., et al. “Regulation of HOXA-10 expression by testosterone in vitro and in the endometrium of patients with polycystic ovary syndrome”. Journal of Clinical Endocrinology and Metabolism 88.1 (2003): 238-243.
  14. Lessey BA., et al. “Aberrant integrin expression in the endometrium of women with endometriosis”. Journal of Clinical Endocrinology and Metabolism 79.2 (1994): 643-649.
  15. Wei Q., et al. “Reduced expression of biomarkers associated with the implantation window in women with endometriosis”. Fertility and Sterility 91.5 (2009): 1686-1691.
  16. Sutherland AE., et al. “Developmental regulation of integrin expression at the time of implantation in the mouse embryo”. Development 119.4 (1993): 1175-1186.
  17. Senturk LM and Arici A. “Leukemia inhibitory factor in human reproduction”. American Journal of Reproductive Immunology 39.2 (1998): 144-151.
  18. Apparao KB., et al. “Elevated endometrial androgen receptor expression in women with polycystic ovarian syndrome”. Biology of Reproduction 66.2 (2002): 297-304.
  19. Donaghay M and Lessey BA. “Uterine receptivity: alterations associated with benign gynecological disease”. Seminars in Reproductive Medicine 25.6 (2007): 461-475.
Citation: Ertan Zan and Bülent Duran. “Laparoscopic Ovarian Drilling Regulates Endometrial LIF and Integrin Expression in PCOS”. EC Endocrinology and Metabolic Research 5.1 (2020): 01-05.

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