Research Article
Volume 7 Issue 10 - 2020
Pepsin as a Biomarker for Self-Diagnosing Reflux Associated Symptoms
Katie H A Boulton*, Jeanine Fisher, Andrew D Woodcock and Peter W Dettmar
RD Biomed Limited, Castle Hill Hospital, Cottingham, UK
*Corresponding Author: Katie H A Boulton, RD Biomed Limited, Castle Hill Hospital, Cottingham, UK.
Received: August 19, 2020; Published: September 15, 2020


Background: An increasing number of individuals are seeking self-diagnosis for their reflux related symptoms in the UK. Many individuals present with troublesome symptoms, such as regurgitation, heartburn and hoarseness and want a quick non-invasive diagnosis. Pepsin is produced in the stomach and is present in the backflow of gastric refluxate, therefore an excellent biomarker for reflux within a saliva sample. The aim of the study was to show how pepsin can be used as a biomarker for reflux, using the non-invasive diagnostic Peptest for the self-diagnosis of reflux related symptoms.

Methods: A total of 739 self-referral individuals [328 males, 411 females] provided three saliva samples for pepsin analysis. The first saliva sample taken ‘on waking’, the other two samples taken either post-prandial or post-symptom. The lateral flow device Peptest was used to detect pepsin in saliva and a PepCube reader used to determine the concentration of pepsin [ng/ml] in the sample.

Results: A total of 739 self-referral individuals provided saliva samples, the mean age of all individuals was 53 years. A total of 489 [66%] individuals had one or more saliva samples pepsin positive with an overall mean pepsin concentration of 205 ng/ml ± 4.8. 250 [34%] of individuals had pepsin negative saliva samples.

Conclusion: Peptest gives individuals access to a non-invasive diagnostic test for self-diagnosing reflux related symptoms, using pepsin as the biomarker for detecting salivary pepsin.

Keywords: Saliva Samples; Pepsin; Biomarker; Reflux Diagnosis; Reflux Symptoms


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Citation: Katie H A Boulton., et al. “Pepsin as a Biomarker for Self-Diagnosing Reflux Associated Symptoms”. EC Gastroenterology and Digestive System 7.10 (2020): 49-56.

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