Research Article
Volume 8 Issue 8 - 2021
Effect of Orally Administered Relaxin in Experimental Nash
Colucci Giuseppe1*, Maggioni M2, Gatti S3 Bianchi A4, Palladini G5, Colombo M6 and Angiolini L4
1UO Gatroenterologia ed Epatologia, Fondazione IRCCS Ca’ Granda Policlinico, Dipartimento di Fisiopatologia e Trapianti, Università degli Studi di Milano, Milan, Italy
2UO Anatomia Patologica, Fondazione IRCCS Ca’ Granda Policlinico, Dipartimento dei Servizi e Medicina Preventiva, Università degli Studi di Milano, Milan, Italy
3Centro di Ricerche Chirurgiche Precliniche, IRCCS Ca’ Granda Policlinico, Milan, Italy
4IBSA Fertility Department, Institute Biochimique, Lugano, Switzerland
5Department of Internal Medicine, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Pavia, Italy
6Translational Research Center in Hepatology, Humanitas Research Center, Rozzano, Italy
*Corresponding Author: Colucci Giuseppe, UO Gatroenterologia ed Epatologia, Fondazione IRCCS Ca’ Granda Policlinico, Dipartimento di Fisiopatologia e Trapianti, Università degli Studi di Milano, Milan, Italy.
Received: June 08, 2021; Published: July 17, 2021




Abstract

Relaxin (RLX), a hormone-like molecule with pleiotropic effects, has been found to reduce matrix deposition and mediate collagen degradation in animal models of progressive liver diseases.

In the present study, we evaluated whether RLX affects the development of liver fibrosis in an experimental mouse model of NASH in C57BL6 male mice fed with a methionine-choline deficient diet (MCD). Mice were treated per os as we intended to assess the enteric absorption and bioavailability of orally administered RLX (RLX purified from pig ovaries, IBSA SA, Lugano, Switzerland). Mice were fed the MCD diet for 6 weeks. After the initial 3 weeks, one group received drinking water supplemented with RLX (25 mcg/ml) whereas the other continued to receive regular water. A third group of mice fed a regular diet served as control. After 3 additional weeks mice were anesthetized and sacrificed.

A significant, reduced expression of the pro-inflammatory cytokines TNF-α and of relevant markers of active fibrogenesis and extracellular matrix deposition, Col1A1 and α-SMA, was observed in mice treated with RLX vs controls. RLX also induced a significant decrease of TIMP1 and an increase of both MMP 2 and 9 when evaluated by zymographic analysis in liver extracts.

These data support the absorption of orally administered RLX and confirm its potential therapeutic use in the management of liver fibrosis.

Keywords: Relaxin; Liver Fibrosis; NAFLD/NASH

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Citation: Colucci Giuseppe., et al. “Effect of Orally Administered Relaxin in Experimental Nash” EC Gastroenterology and Digestive System 8.8 (2021): 11-20.

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