Mini Review
Volume 10 Issue 8 - 2021
The Benefits of Density Gradient Centrifugation for Sperm Preparation for ICSI, as Well as the Many Forms of Density Gradient Preparation and their Applications in Bacteriospermia and Contaminated Samples Study in Male and Female Infertile Couples
Bezar Ghan VV*, Deepthi, Vineeth Reddy Lekkala, Geetha Reddy, Ram Reddy and Raja Gopal K
Amrutha Fertility Centre, Yenapoya Medical College, Yenapoya University, India
*Corresponding Author: Bezar Ghan VV, Amrutha Fertility Centre, Yenapoya Medical College, Yenapoya University, India.
Received: June 22, 2021; Published: July 20, 2021




Abstract

Human sperm is very diverse, varying not just from person to person but also from sample to sample. According to the World Health Organization, spermatozoa quality is determined by the motility, concentration, and shape of the sperm in each given sample (WHO).

Keywords: Density Gradient; ICSI; Bacteriospermia

References

  1. Sakkas D., et al. “The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies”. Human Reproduction5 (2000): 1112-1116.
  2. Muratori M., et al. “Sperm selection with density gradient centrifugation and swim up: effect on DNA fragmentation in viable spermatozoa”. Scientific Reports1 (2019): 1-12.
  3. Natali I. “Sperm preparation techniques for artificial insemination-comparison of sperm washing, swim up, and density gradient centrifugation methods”. Dalam: Manafi M, penyunting. Artificial insemination in farm animals. In Tech Open (2011): 115-122.
  4. Price CA. “Centrifugation in density gradients”. Academic Press (2014).
  5. Zhang Q and Sun X. “Density Gradient Ultracentrifugation Technique”. In Nanoseparation Using Density Gradient Ultracentrifugation (2018): 37-58.
  6. Astarto NW., et al. “Comparison between two-layer density gradient and three-layer density gradient technique for sperm preparation at aster fertility clinic, Dr. Hasan Sadikin General Hospital”. International Journal of Integrated Health Sciences 1 (2014): 40-44.
  7. Sbracia M., et al. “Sperm function and choice of preparation media: comparison of Percoll and Accudenz discontinuous density gradients”. Journal of Andrology 1 (1996): 61-67.
  8. Inoue O., et al. “Clinical efficacy of a combination of Percoll continuous density gradient and swim-up techniques for semen processing in HIV-1 serodiscordant couples”. Asian Journal of Andrology 2 (2017): 208.
  9. Henzy J. “Measuring up”. Evolution, Odds and Ends, Teachers Corner, Permalink (2015).
  10. Litke E. “Fact check: No, N95 filters are not too large to stop COVID-19 particles”. USA TODAY Newspaper (2020).
  11. Henkel RR and Schill WB. “Sperm preparation for ART”. Reproductive Biology and Endocrinology1 (2003): 108.
Citation: Bezar Ghan VV., et al. “The Benefits of Density Gradient Centrifugation for Sperm Preparation for ICSI, as Well as the Many Forms of Density Gradient Preparation and their Applications in Bacteriospermia and Contaminated Samples Study in Male and Female Infertile Couples”. EC Gynaecology 10.8 (2020): 56-59.

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