Research Article
Volume 18 Issue 9 - 2022
An Investigation into the In Vitro Antibacterial Activity of Moringa Leaf and Seed Extract
Yasser Jabah Alsendi*, Vicki Anderson and Saeid Al Matif
Department of Pharmacy, Liverpool John Moores University, Saudi Arabia
*Corresponding Author: Yasser Jabah Alsendi, Department of Pharmacy, Liverpool John Moores University, Saudi Arabia.
Received: December 22, 2020; Published: August 12, 2022




Abstract

Aim: Investigate the antibacterial activity of aqueous and alcoholic (ethanol) extracts of Moringa leaves and seeds on Escherichia coli and Staphylococcus aureus using Kirby-Bauer antimicrobial Disk diffusion susceptibility assays.

Materials and Methods: Water and ethanol extractions of ground Moringa seed and ground Moringa leaf were prepared and compared against ethanol alone as well as antibiotic treatments in Kirby-Bauer assays against E. coli and S. aureus. Gentamycin was used as a positive control antibiotic against E. coli and Ciprofloxacin was used for S. aureus.

Key Findings: ANOVA for treatments against E. coli and S. aureus showed there were statistically significant differences between treatment groups: (F(5,48) = 120.10, p = 0.000). and (F(5,48) = 176.69, p = 0.000 respectively. Water extraction of Moringa seed showed the highest level of antibacterial activity, and in contrast there was no statistical significant difference between the ethanol extractions of Moringa seed and leaf versus ethanol alone.

Conclusion: The results confirm that there are compounds in Moringa seed and leaf that have antibacterial activity. It is likely that these compounds are hydrophilic as suggested by the water extractions showing the most inhibition compared to ethanol extractions. Further studies are needed to identify the specific compounds most responsible for the antibacterial activity found which could lead to new antibiotics being developed.

Keywords: Antibacterial Activity; Moringa Leaf and Seed; Escherichia coli; Staphylococcus aureus

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Citation: Yasser Jabah Alsendi., et al. “An Investigation into the In Vitro Antibacterial Activity of Moringa Leaf and Seed Extract”. EC Microbiology 18.9 (2022): 01-15.

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