Research Article
Volume 18 Issue 7 - 2022
Changes in the Levels of CD4+ T-Cell Count, 8-Hydroxy-2-Deoxyguanosine, Total Dopamine, Cortisol and Neopterin at Different Stages of HIV1-Infection
Moses Akiibinu1*, Fola Tayo2, John Maduabuchi1, Titiloye Oyewumi3, Timothy Olagunju4 and Bunmi Kotun5
1Department of Biochemistry and Chemistry, Caleb University Lagos, Nigeria
2Pharmaco-Herbal Development Laboratory, Famot Health Care Ltd, Iyeri Estate, Ikorodu-Itoikin Road, Imota, Lagos State, Nigeria
3Immunology Unit, Department of Chemical Pathology, College of Medicine, University of Ibadan, Ibadan, Nigeria
4Department of Chemical Pathology, University College Hospital, Ibadan, Nigeria
5Department of Biological Science and Industrial Biotechnology, Ccaleb University Lagos, Nigeria
*Corresponding Author: Moses Akiibinu, Department of Biochemistry and Chemistry, Caleb University Lagos, Nigeria.
Received: May 24, 2022; Published: June 13, 2022




Abstract

Background: CD4 + T-cell remains the golden biomarker that changes with progression/severity of HIV/AIDS. This study assessed the levels of certain selected metabolites in different stages of HIV1- infection.

Methods: Sixty newly diagnosed HIV1-infected patients with varied CD4 + T-cell counts participated in this study. They were classified as CD4 + T-cell counts > 250 cells/µl (group 1), CD4 + T-cell counts ranging from 180 to 250 cells/µl (group2), and CD4 + T-cells < 180 cells/µl (group3). Another 25 apparently healthy age and sex-matched individuals without HIV1-infection served as controls (group4). Plasma levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), total dopamine (TD), cortisol and neopterin were determined in all participants using enzyme linked immunosorbent assay (ELISA) methods. CD4+T-cell count was determined using flow cytometry method.

Results: Significantly (p < 0.05) higher levels of 8-OHdG, TD and neopterin were observed in all groups compared with controls. There were no significant (p > 0.05) changes in the levels of cortisol in all groups compared with controls. There was a significant correlation (r = -0.49, p < 0.05) between TD and CD4 + T-cell in HIV1-infected patients. Neopterin, cortisol and 8-OHdG did not show any significant (r = -0.14, p > 0.05; r = -0.11, p > 0.05; r = -0.25, p > 0.05 respectively) correlations with CD4 + T-cells in HIV-1 infected patients.

Conclusion: HIV1 infection could induce oxidative DNA-damage, dopamine synthesis and macrophage activation. Total dopamine level could be used to assess the progression of HIV1-infection. Further investigation may be needed to ascertain the findings.

 

Keywords: DNA-Damage; Dopamine; Cortisol; Macrophage Activation; HIV1; CD4-T-Cells

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Citation: Moses Akiibinu., et al. “Changes in the Levels of CD4+ T-Cell Count, 8-Hydroxy-2-Deoxyguanosine, Total Dopamine, Cortisol and Neopterin at Different Stages of HIV1-Infection”. EC Microbiology 18.7 (2022): 01-12.

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