Research Article
Volume 18 Issue 9 - 2022
Molecular Characterization of Multi Drug Resistant Bacteria Isolated from Diabetic Foot Infections in Jabir Abolez Diabetic Foot Center - Khartoum - Sudan
Samia S Mohamed Ismail1*, Elsheikh Mahgoub2, Khanssa M Elamin3 and Husham M Taha Aloob4
1Department of Clinical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Dongola University, Sudan
2Council of Clinical Microbiology and Infectious Diseases, Sudan Medical Specialization Board, Sudan
3Vesion and Endocrinologist Jabir Abolez Diabetic Foot Center, Khartoum, Sudan
4Department of Microbiology and Parasitology, Faculty of Medical Laboratory Sciences, Dongola University, Sudan
*Corresponding Author: Samia S Mohamed Ismail, Department of Clinical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Dongola University, Sudan.
Received: July 01, 2022; Published: August 18, 2022


Background: Diabetic foot ulcers (DFU) are among major health problems which impact the socio-economic burden globally. Multi-drug resistance has become a major problem for the treatment of pathogenic bacterial infections.

Objective: The present study was aimed to determine the molecular characterization of multi drug resistance bacteria isolated from diabetic foot Infections patients with septic foot infection attending Jaber Abolez Diabetic Center from the 1st of February till the 31st of July 2021.

Methods: This descriptive cross-sectional study. Wound swabs were collected from each wound after the wound had been cleansed and debrided. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion and B-lactamase genes were detected used PCR.

Results: The most common isolated bacteria were Proteus mirabilis (80.0% of cases) followed by Enterococcus faecalis (63% of cases) and Staphylococcus aureus (36% of cases). The isolated bacteria were 43% aerobic Gram negative, 21% aerobic gram positive, 19% anaerobic gram positive and anaerobic gram negative. Most of the isolated bacteria were resistant to multiple antibiotics especially for ciprofloxacin (100% of the isolated bacteria) and cephalosporins and ampicillin (98% of the isolated bacteria). The lowest cases of resistance were to meropenem. The commonest multi-drug resistance gram negatives of the B-lactamase genes using PCR was extended spectrum (ESBL) bla SHV which detected in 83% of isolated gram-negative bacteria followed by extended spectrum (ESBL) bla CTXM-1 and Carbapenemase gene bla NDM; 66% and 61% respectively.

Conclusion: Multidrug-resistant facultative anaerobic bacteria are overrepresented as agents of DFU. High rates of resistance toward most of the tested antibiotics were reported. Gram-negative organisms expressed high rates of extended spectrum B-lactamase genes. BlaSHV was the most detected gene.

Keywords: Bacteria; Diabetic Foot; Multi Drug Resistance; PCR; β-Lactamase; Sudan


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Citation: Samia S Mohamed Ismail., et al. “Molecular Characterization of Multi Drug Resistant Bacteria Isolated from Diabetic Foot Infections in Jabir Abolez Diabetic Foot Center - Khartoum - Sudan”. EC Microbiology 18.9 (2022): 06-18.

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