Research Article
Volume 3 Issue 2 - 2021
Human Herpesvirus 4 or Epstein-Barr Virus Infections and Putative Role in the Cause of Childhood Leukemia
Haitham E Elawad1*, Esraa M Abdalla1, Mohammed EH Ournasseir1 and Yassir Fadl Abubakar2
1Department of Microbiology, Medical Laboratories Sciences, Omdurman Islamic University, Omdurman, Sudan
2Department of Microbiology, Immunology & Parasitology, Faculty of Medicine and Health Sciences, Gadarif university, Omdurman, Sudan
*Corresponding Author: Haitham E Elawad, Department of Microbiology, Medical Laboratories Sciences, Omdurman Islamic University, Omdurman, Sudan.
Received: July 17, 2020; Published: January 20, 2021


Background: The exact cause of acute lymphoblastic leukemia (ALL), a severe cancer in Sudan, is still under assessment. Substantial progress has been made in understanding the contribution of infections to the aggressive transformation of B-cells, where Epstein-Barr virus (EBV) has indeed been reported as transformative triggers. The identity of an infectious agent remained anonymous. As there is no specific candidate agent in sight, we assumed that the EBV might be implicated in the underlying cause of leukemia.

Methods: A total of 90 participants were recruited for this study. Of these, 42 were ALL pediatric leukemia patients, 8 were positive-control patients with EBV-associated lymphoid malignancies and 40 were healthy individuals. They were tested for EBV-IgG antibodies. Only positive samples were detected by the PCR targeting the EBV BamH1W region.

Results: Tests have demonstrated the detectability of EBV DNA in a number of leukemia and control patients. Almost all subjects had EBV-IgG positive (> 85% of cases and controls) whereas EBV DNA was detected in 20% of cases (N = 42) and was not found in controls.

Conclusion: Our conclusion stated that there was no EBV DNA in all leukemic cells, and therefore most likely infection did not lead to ALL in Sudanese children.

Keywords: EBV; PCR; Leukemia; Diagnosis; BamH1W Region


  1. Kugelberg E. “Tumour immunology: Malaria alters B cell lymphomagenesis”. Nature Reviews Immunology9 (2015): 528.
  2. Foster LH and CA Portell. “The role of infectious agents, antibiotics, and antiviral therapy in the treatment of extranodal marginal zone lymphoma and other low-grade lymphomas”. Current Treatment Options in Oncology 6 (2015): 8.
  3. Greaves MF and FE. “Alexander, An infectious etiology for common acute lymphoblastic leukemia in childhood?” Leukemia3 (1993): 349-360.
  4. Kinlen LJ. “Epidemiological evidence for an infective basis in childhood leukaemia”. British Journal of Cancer 1 (1995): 1-5.
  5. Bartenhagen C., et al. “Infection as a cause of childhood leukemia: virus detection employing whole genome sequencing”. Haematologica 5 (2017): e179-e183.
  6. Sehgal S., et al. “High incidence of Epstein Barr virus infection in childhood acute lymphocytic leukemia: a preliminary study”. Indian Journal of Pathology and Microbiology 1(2010): 63-67.
  7. Martin-Lorenzo A., et al. “Infection Exposure is a Causal Factor in B-cell Precursor Acute Lymphoblastic Leukemia as a Result of Pax5-Inherited Susceptibility”. Cancer Discovery 12 (2015): 1328-1343.
  8. Cohen JI. “Epstein-Barr virus infection”. The New England Journal of Medicine 7 (2000): 481-492.
  9. Haitham E Elawad., et al. “The Possible Involvement of Epstein -Barr virus In the Etiology of Leukemia”. Medical Microbiology and Diagnosis1 (2014).
  10. Ahmed HG OS and Ashankyty IM. “Incidence of Epstein-Barr Virus in Pediatric Leukemia in the Sudan”. 12.2 (2012): 79-150.
  11. Calvente I., et al. “Exposure to electromagnetic fields (non-ionizing radiation) and its relationship with childhood leukemia: a systematic review”. Science of the Total Environment 16 (2010): 3062-3069.
  12. Corso A., et al. “Chronic myelogenous leukemia and exposure to ionizing radiation--a retrospective study of 443 patients”. Annals of Hematology 2 (1995): 79-82.
  13. Austin H., et al. “Benzene and leukemia. A review of the literature and a risk assessment”. American Journal of Epidemiology 3 (1988): 419-439.
  14. Arico M., et al. “Acute lymphoblastic leukemia and Down syndrome: presenting features and treatment outcome in the experience of the Italian Association of Pediatric Hematology and Oncology (AIEOP)”. Cancer 3 (2008): 515-521.
  15. A E Greijer., et al. “Quantitative multi-target RNA profiling in Epstein-Barr virus infected tumor cells”. Journal of Virological Methods 241 (2017): 24-33.
  16. Kim KY., et al. “Current State of PCR-Based Epstein-Barr Virus DNA Testing for Nasopharyngeal Cancer”. Journal of the National Cancer Institute 4 (2017).
  17. Julie L Ryan., et al. “Epstein-Barr Virus Quantitation by Real-Time PCR Targeting Multiple Gene Segments”. The Journal of Molecular Diagnostics 4 (2004): 378-385.
  18. Sanosyan A., et al. “The impact of targeting repetitive BamHI-W sequences on the sensitivity and precision of EBV DNA quantification”. PLoS One8 (2017): e0183856.
  19. Vazquez-Guillen JM., et al. “Infection and coinfection by human papillomavirus, Epstein-Barr virus and Merkel cell polyomavirus in patients with squamous cell carcinoma of the larynx: a retrospective study”. Peer Journal 6 (2018): e5834.
  20. Zhou T., et al. “Associations between environmental factors and serological Epstein-Barr virus antibodies in patients with nasopharyngeal carcinoma in South China”. Cancer Medicine 10 (2019): 4852-4866.
  21. Yu KJ., et al. “Prognostic utility of anti-EBV antibody testing for defining NPC risk among individuals from high-risk NPC families”. Clinical Cancer Research 7 (2011): 1906-1914.
  22. Lei K., et al. “Quantitative analysis of circulating cell-free Epstein-Barr virus (EBV) DNA levels in patients with EBV-associated lymphoid malignancies”. British Journal of Haematology 111 (2000): 239-246.
Citation: Haitham E Elawad., et al. “Human Herpesvirus 4 or Epstein-Barr Virus Infections and Putative Role in the Cause of Childhood Leukemia”. EC Nursing and Healthcare 3.2 (2021): 55-61.

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