Research Article
Volume 17 Issue 5 - 2022
Extraction of Oil from Fig-Leaf Gourd Seed (Cucurbita ficifolia) Using Enzyme-Assisted Three-Phase Partitioning Method and Evaluation of Quality Parameters of the Final Oil
Anh TN Le and Tuan Q Dang*
Department of Food Technology, International University-VNU-HoChiMinh City, Vietnam
*Corresponding Author: Tuan Q Dang, Department of Food Technology, International University (VNU-HCMC), Thu Duc City, Ho Chi Minh City, Vietnam.
Received: March 29, 2022; Published: April 22, 2022


This study was aimed at the effect of enzymatic treatment on the oil yield in enzyme assisted three-phase partitioning (EATPP) extraction of oil from fig-leaf gourd seeds (Cucurbita ficifolia). Two types of enzymes (cellulase/protease) were assessed for their effectiveness in oil release from the seeds. Several parameters in enzymatic treatment, such as enzyme concentration, incubation temperature, and treatment duration were studied. The optimum conditions were chosen based on the highest oil yield extracted by EATPP. In details, the yield of oil extracted by common Soxhlet method (control) and by TPP without enzyme (blank) were 46.63% and 31.17%, respectively. Meanwhile, for the Celluclast®, the highest oil yield of 37.50% was achieved in the conditions of 0.5% (v/w of dried matter) enzyme concentration, at 40°C within 2h of incubation; for Alcalase®, it was 41.0%, achieved in the conditions of 1.5% (v/w of dried matter) enzyme concentration, at 50°C within 3h of incubation. Apparently, the oil yield from Soxhlet extraction was significantly higher than from EATPP. However, the oils obtained by EATPP were of higher quality than that extracted by Soxhlet method, in terms of total phenolic content, total flavonoid content, radical scavenging activity, peroxide value and free fatty acid values. Application of enzymes in TPP process for fig-leaf gourd seed helped to increase the extraction efficiency and obtain oil of good quality.

Keywords: Cucurbita ficifolia; Enzyme-Assisted Extraction; Oil Quality; Oil Yield; Three Phase Partitioning


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Citation: Anh TN Le and Tuan Q Dang. “Extraction of Oil from Fig-Leaf Gourd Seed (Cucurbita ficifolia) Using Enzyme-Assisted Three- Phase Partitioning Method and Evaluation of Quality Parameters of the Final Oil”. EC Nutrition 17.5 (2022): 10-21.

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