Research Article
Volume 12 Issue 7 - 2021
Bone Marrow-Derived Mesenchymal Stem Cells Reduce Glycosaminoglycan Deposition in Deep Digital Flexor Collagenase-Induced Tendinopathy
Sherry A Johnson1, John D Lutter2, Robert K Schneider3, Elizabeth W Biscoe4, Gregory D Roberts5, Julie A Cary5 and David D Frisbie1*
1Department of Clinical Sciences, Orthopaedic Research Center at the C. Wayne McIlwraith Translational Medicine Institute, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA
2Kansas State University, Veterinary Health Center, Manhattan, KS, United States
3Schneider and Stenslie Equine, Auburn, WA, USA
4Animal Imaging, Irving, TX, USA
5Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, United States
*Corresponding Author: David D Frisbie, Department of Clinical Sciences, Orthopaedic Research Center at the C. Wayne McIlwraith Translational Medicine Institute, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
Received: May 13, 2021; Published: June 08, 2021




Abstract

Intrathecal deep digital flexor tendon (DDFT) injuries are a common source of distal limb lameness in athletic horses with prognosis considered guarded to poor. The ability of mesenchymal stem cells to improve intrathecal DDFT healing is difficult to objectively evaluate in the clinical setting due to inherent variations in extent, severity, configuration and chronicity of naturally occurring lesions. The study objective was to evaluate the effects of intra-lesional mesenchymal stem cells compared to untreated controls in a collagenase model of intrathecal deep digital flexor tendinopathy. Tendinopathy of both forelimb DDFTs within the pastern region was induced with collagenase. One randomly assigned limb was treated with intra-lesional MSC injection with the opposite limb serving as untreated control. Horses were placed into a controlled exercise program with ultrasonographic and magnetic resonance imaging evaluations at 0, 14-, 60-, 90-, and 214-days post treatment. Post-mortem histologic and biochemical analysis was performed. Inflammatory cellular infiltration and glycosaminoglycan content were significantly lower in MSC treated tendons (P = .05, P = .002, respectively). The results herein demonstrate that the use of MSCs significantly lessened the amount of inflammatory cellular infiltrate and glycosaminoglycan content in enzymatically damaged intrathecal DDF tendon cells (130% and 42% reductions, respectively). Only subtle differences in ultrasonographic or MRI imaging characteristics were appreciated, leading authors to speculate that physiologic effects of MSCs may not manifest as imaging-apparent differences. The use of MSCs in collagenase induced DDF tendinopathy is associated with chronic tendon enlargement, improved glycosaminoglycan content and a decreased inflammatory response.

 

Keywords: Tendinopathy; Horse; Collagenase

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Citation: David D Frisbie., et al. “Bone Marrow-Derived Mesenchymal Stem Cells Reduce Glycosaminoglycan Deposition in Deep Digital Flexor Collagenase-Induced Tendinopathy”. EC Orthopaedics 12.7 (2021): 18-32.

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