Research Article
Volume 6 Issue 4 - 2021
In Vitro Evaluation of Eucalyptus pellita Essential Oil against Varroosis in Apis mellifera
Osmaida Estrada Cutiño1*, José Alfredo Guevara Franco2, Lucía Fuentes Jiménez3, Harold Font Puentes1, Enrrique Albis Zayas Bazán4, Yeny Tamayo Moreno1 and Carlos Olmo González1
1Department Veterinary Medicine, University of Granma, Cuba
2Academic Department of Animal Science and Habitat Conservation (DACACH). Universidad Autónoma de Baja California Sur (UABCS). México
3Higher Technological Institute of the East of the State of Hidalgo (ITESA). México
4Empresa Apícola Granma, Cuba
*Corresponding Author: Osmaida Estrada Cutiño, Department Veterinary Medicine, University of Granma, Cuba.
Received: February 25, 2021; Published: March 16, 2021




Abstract

In order to evaluate in vitro the essential oil of Eucalyptus pellita against varroosis in Apis mellifera, fresh leaves were randomly collected from Eucalyptus plants. The essential oil extraction was carried out by means of the hydrodistillation method (with water and steam) at the Center for the Study of Applied Chemistry, University of Granma. The parasites were collected in an apiary located in the Santa Isabel community of the Bayamo municipality from zanganero combs. For the evaluation of the acaricidal effect, concentrations of 25, 50, 75 and 100% were used respectively. Distilled water was used as a control. 20 insects were placed in a Petri dish with filter paper impregnated with the product, less in the control. Readings were made at 4, 8 and 24 hours. The percentage of mortality of the mites was determined. The analysis of variance was carried out for the comparison of proportions. Through the use of the COMPARPRO 1.0 Program [4]. The results show greater antiparasitic activity when using the 75 and 100% concentrations during 24 hours of investigation. It is concluded that the biopreparation has an antiparasitic effect against Varroa destructor of Apis mellifera in vitro.

Keywords: Oil; Essential; Eucalyptus pellita; Varroa

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Citation: Osmaida Estrada Cutiño., et al."In Vitro Evaluation of Eucalyptus pellita Essential Oil against Varroosis in Apis mellifera”. EC Veterinary Science 6.4 (2021): 24-30.

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