Research Article
Volume 6 Issue 6 - 2021
Comparison of Two Different Measurement Techniques and Three Sample Materials for Bovine Haptoglobin in Early Postpartum Holstein Friesian Dairy Cows
Ruth Schmitt1*, Rudolf Staufenbiel1 and Laura Pieper2
1Ruminant and Swine Clinic, Free University of Berlin, Germany
2Institute for Veterinary Epidemiology and Biostatistics, Free University of Berlin, Germany
*Corresponding Author: Ruth Schmitt, Ruminant and Swine Clinic, Free University of Berlin, Germany.
Received: May 11, 2021; Published: May 27, 2021




Abstract

This research was conducted to compare two measurement techniques and three sample materials for the analysis of bovine haptoglobin (Hp), an acute-phase protein, that can potentially be used to detect excessive postpartum inflammation in dairy cows on both farm and individual levels. Serum Hp concentrations of 99 fresh lactating Holstein Friesian dairy cows from 10 farms in the northeastern region of Germany were assessed using both pooled and individual serum samples. A colorimetric measurement on a chemical autoanalyzer (CA) was compared to an enzyme-linked immunosorbent assay (ELISA)-test kit. Additionally, Hp levels in pooled serum, lithium heparinized (LH) plasma and ethylenediaminetetraacetic acid (EDTA) plasma samples were compared. Mean serum Hp levels varied significantly between farms. Pooled serum samples showed high correlations with the mean of individual samples. Hp (ELISA) concentrations were on average 40% lower than Hp (CA) concentrations. Dilutions interfered with the analytical precision of the ELISA. Compared to serum, Hp (CA) concentrations were 2.5 and 2.3 times higher in LH plasma and EDTA plasma, respectively, which was not observed with the ELISA. In conclusion, Hp differentiates farms regarding the inflammatory state of transition cows. Pooled samples of farms can be used for this purpose. Laboratories should specify different reference values for Hp when using colorimetric measurements on a CA or ELISA-test kits, respectively. Furthermore, dilutions need to be prepared with maximum precision. Finally, serum should be the preferred sample type for colorimetric Hp measurements.

 

Keywords: Acute-Phase Protein; Haptoglobin; Transition Dairy Cow; Inflammation; Clinical Laboratory Diagnosis

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Citation: Ruth Schmitt., et al.“Comparison of Two Different Measurement Techniques and Three Sample Materials for Bovine Haptoglobin in Early Postpartum Holstein Friesian Dairy Cows”. EC Veterinary Science 6.6 (2021): 10-21.

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