Research Article
Volume 6 Issue 7 - 2021
Development of a New Transport Box for Chilled Stallion Semen, ECOOL Box
Charlène Rouillon1*, Guy Delhomme1*, Frederic de Geoffroy2, Clothilde Gourtay2 and Eric Schmitt1
1IMV Technologies, Z.I. N°1 Est, Saint-Ouen-Sur-Iton, France
2Jumenterie du Haras du Pin, Institut Français du Cheval et de l’Equitation, Exmes, France
*Corresponding Author: Charlène Rouillon and Guy Delhomme, IMV Technologies, Z.I. N°1 Est, Saint-Ouen-Sur-Iton, France.
Received: June 07, 2021; Published: June 24, 2021


Today, doses of refrigerated stallion semen are stored, sent, and transported in single-use polystyrene transport boxes. To address a growing concern for ecological and sustainable development, a new transport box made of recycled material, eco-responsible, called ECOOL Box, has been developed to ensure the transport and conservation of doses. Thermal performance to maintain the temperature between + 2°C and + 15°C for 48 hours, cooling kinetics during the first hour of storage, and biological parameters relating to motility, velocity and sperm viability were analyzed and compared to a control box. In total, up to 10 ejaculates were diluted, packaged in syringes (20.106 spermatozoa/mL, 10 mL per dose) and stored in transport boxes for 48 hours. The boxes were placed in a thermal chamber mimicking the daily temperature variations during a temperate winter or summer climate. Temperature readings were taken every 30 seconds using thermocouples. The quality of the semen was analyzed by a computer-assisted semen analyzer at the day of collection and after the 48 hours of storage. Sperm viability at the end of the 48 hours was analyzed using flow cytometry. No significant difference between the two boxes was observed on all thermal and biological parameters. The ECOOL Box allows the cooling of semen doses at a rate of - 0.13°C/min, with no detrimental effect. In winter conditions, the ECOOL Box allows to maintain the doses between + 2.7°C and + 4.0°C for 48 hours. In summer condition, the ECOOL Box maintains the doses between + 7.2°C and + 15°C for 42 hours. The total sperm motility is reduced by only 5% compared to the day of collection. However, it remains above 70% in both conditions at the end of the experiment, which is in line with the recommendations of the French National Studs. The ECOOL Box thus shows its thermal and biological efficiency and allows the transport of equine doses in a safe and eco-responsible way.


Keywords: Stallion; Chilled Semen; Transport; Motility; Viability; Temperature


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Citation: Charlène Rouillon., et al. “Development of a New Transport Box for Chilled Stallion Semen, ECOOL Box”. EC Veterinary Science 6.7 (2021): 41-52.

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