Research Article
Volume 6 Issue 7 - 2021
Effects of Calcium Supplementation on the Histology of the Cardiac Myocytes of Albino Wistar Rats
Hope Avwerhosuo Ugbero Odogun1*, Ighoyinvwin Peter Odogun2 and Olufunke Omolola Arishe1
1Department of Physiology, University of Benin, Nigeria
2Department of Microbiology, Near East University, Cyprus
*Corresponding Author: Hope Avwerhosuo Ugbero Odogun, Department of Physiology, University of Benin, Benin City, Edo State, Nigeria.
Received: April 24, 2021; Published: June 25, 2021


We studied the effects of calcium supplementation on cardiac myocytes of albino wistar rats. A total of 20 albino wistar rats were purchased and acclimatized for 2 weeks and were served regular rat food and drank tap water ad libitum. They were then randomly assigned to four groups of five animals each. Group A (control) received a placebo; Group B received a low dose (7.69 mg/kg) of calcium supplements; Group C received a moderate dose (15.38 mg/kg) of calcium supplements; Group D received a high dose (23.07 mg/kg) of calcium supplements. Calcium supplements were administered for 28 consecutive days after which the animals were euthanized under chloroform anesthesia and heart was removed for histological processing and microscopic examination. Blood samples were also collected for estimation of serum calcium concentration. Results obtained showed no difference in the serum calcium concentrations amongst the groups. The histology of the heart showed normal histology in control and low dose groups and degenerative changes in the moderate and high dose groups. It was thus concluded that calcium supplementation in moderate and high doses may lead to degeneration of the cardiac myocytes.


Keywords: Calcium Supplementation; Cardiac Myocytes; Degeneration; Myocardial Infarction; Apoptosis; Histology


  1. Marshall WJ. “Clinical Chemistry (3rd edition)”. Mosby: London (1995): 97-102.
  2. NIH Consensus Development Panel on Optimal Calcium Intake. “Optimal calcium intake. NIH Consensus Conference”. Journal of the American Medical Association 272 (1994): 1942-1948.
  3. Criscione, J.C., et al. “The myocardial band: simplicity can be a weakness”. European Journal of Cardio-Thoracic Surgery 28 (2005): 363-364.
  4. Reid IR., et al. “Effects of calcium supplementation on body weight and blood pressure in normal older women: a randomized controlled trial”. The Journal of Clinical Endocrinology and Metabolism 90 (2005): 3824-3829.
  5. Pittas AG., et al. “The Effects of Calcium and Vitamin D Supplementation on Blood Glucose and Markers of Inflammation in Non-diabetic Adults”. Diabetes Care4 (2007): 980-986.
  6. Jahangir M. “Anatomy and Physiology for Health Professionals”. Jones and Bartlett Publishers (2011): 213.
  7. Corno AF., et al. “Inter-ventricular septum: New observations on the structure and function coupling”. Basic Applied Myology 19 (2009): 41-48.
  8. Gouda ZA., et al. “Histological architecture of cardiac myofibers composing the left ventricle of murine heart”. Journal of Histology and Histopathology2 (2)015: 2-5.
  9. Torrent-Guasp F., et al. “Towards new understanding of the heart structure and function”. European Journal of Cardio-Thoracic Surgery 27 (2005): 191-201.
  10. Deng D., et al. “An image-based model of the whole human heart with detailed anatomical structure and fiber orientation”. Computational and Mathematical Methods in Medicine (2012): 870.
  11. Greenbaum RA., et al. “Left ventricular fiber architecture in man”. British Heart Journal 45 (1981): 248-263.
  12. Pentti K., et al. “Use of calcium supplements and the risk of coronary heart disease in 52-62-year-old women: The Kuopio Osteoporosis Risk Factor and Prevention Study”. Maturitas 63 (2009): 73-78.
  13. Reid IR., et al. “Cardiovascular complications of calcium supplements”. Journal of Cellular Biochemistry 116 (2015): 494-501.
  14. Reid IR., et al. “Cardiovascular effects of calcium supplementation”. Osteoporosis International 22 (2011): 1649.
  15. Barrett-Connor E., et al. “Coffee-associated osteoporosis offset by daily milk consumption”. The Journal of the American Medical Association 271 (1994): 280-283.
  16. Balesaria S., et al. “Human duodenum responses to vitamin D metabolites of TRPV6 and other genes involved in calcium absorption”. American Journal of Physiology6 (2009): 1193-1197.
  17. Committee to Review Dietary Reference Intakes for Vitamin D and Calcium, Food and Nutrition Board, Institute of Medicine. Dietary Reference Intakes for Calcium and Vitamin D. National Academy Press. Washington, DC (2010).
  18. Heaney RP., et al. “Calcium absorption in women: relationships to calcium intake, oestrogen status, and age”. Journal of Bone and Mineral Research 4 (1989): 469-475.
  19. Weaver CM., et al. “Choices for achieving adequate dietary calcium with a vegetarian diet”. The American Journal of Clinical Nutrition 70 (1999): 543S-548S.
  20. Kirstetter JE., et al. “The impact of dietary protein on calcium absorption and kinetic measures of bone turnover in women”. Journal of Clinical Endocrinology and Metabolism1 (2005): 26-31.
  21. Heaney RP. “Bone mass, nutrition, and other lifestyle factors”. Nutrition Reviews 54 (1996): S3-S10.
  22. Heaney RP and Rafferty K. “Carbonated beverages and urinary calcium excretion”. The American Journal of Clinical Nutrition 74 (2001): 343-347.
  23. Fenton TR., et al. “Meta-analysis of the quantity of calcium excretion associated with the net acid excretion of the modern diet under the acid-ash diet hypothesis”. The American Journal of Clinical Nutrition4 (2008): 1159-1166.
  24. Abrams SA., et al. “Stable isotopic measurement of endogenous fecal calcium excretion in children”. Journal of Pediatric Gastroenterology and Nutrition4 (1991): 469-473.
  25. Kevin F. “Foley and Lorenzo Boccuzzi (2010). Urine Calcium: Laboratory measurement and clinical utility”. Laboratory Medicine11 (2010): 686.
  26. Diem K and Lenter C. “Scientific Tables”. 7th Ciba-Geigy Limited Basel (2010): 565.
  27. Blaine J., et al. “Renal control of calcium, phosphate, and magnesium homeostasis”. Clinical Journal of the American Society of Nephrology7 (2015): 1257-1272.
  28. Brini M., et al. “Metal Ions in Life Sciences”. In Sigel, A. and Helmut, R.K edition. Springer (2013): 81-137.
  29. Guide for the care and use of laboratory animals.
  30. Ajibade AJ., et al. “Some Morphological Findings on the Heart of Adult Wistar Rats Following Experimental Artesunate Administration”. Current Research in Cardiovascular Pharmacology 1 (2012): 1-9.
  31. Filho HG., et al. “Experimental model of myocardial infarction”. Brazilian Journal of Cardiovascular Surgery 3 (2011): 24-28.
  32. Rautiainen S., et al. “The role of calcium in the prevention of cardiovascular disease-a review of observational studies and randomized clinical trials”. Current Atherosclerosis Reports 15 (2013): 362.
  33. Leopold JA. “Vascular calcification: mechanisms of vascular smooth muscle cell calcification”. Trends in Cardiovascular Medicine 25 (2015): 267-274.
  34. Anderson JJ and Klemmer PJ. “Risk of high dietary calcium for arterial calcification in older adults”. Nutrients 5 (2013): 3964-3974.
  35. Wang X., et al. “Dietary calcium intake and mortality risk from cardiovascular disease and all causes: a meta‐analysis of prospective cohort studies”. BMC Medicine 12 (2014): 158.
  36. Yeager JC and Whitehurst ME. “Verapamil prevents isoproterenol induced cardiac failure in the rat”. Life Science 3 (1982): 299-306.
  37. Bloom S and Davis DL. “Calcium as mediator of isoproterenol induced myocardial necrosis”. American Journal of Pathology 3 (1972): 459-470.
  38. Bhagat B., et al. “cAMP activity and isoproterenol-induced myocardial injury in rats”. Recent Advances in Studies on Cardiac Structure and Metabolism 12 (1976): 465-470.
  39. Singal PK., et al. “Role of free radicals in catecholamine-induced cardiomyopathy”. Canadian Journal of Physiology and Pharmacology11 (1982): 1390-1397.
  40. Vanessa PM., et al. “Myocyte apoptosis in heart failure”. Cardiovascular Research1 (2005): 21-29.
  41. Sabbah HN. “Apoptotic cell death in heart failure”. Cardiovascular Research 45 (2000): 704-712.
  42. Borgers M., et al. “Spotlight on: apoptosis”. Cardiovascular Research 45 (2000): 525-804.
Citation: Hope Avwerhosuo Ugbero Odogun., et al. “Effects of Calcium Supplementation on the Histology of the Cardiac Myocytes of Albino Wistar Rats”. EC Veterinary Science 6.7 (2021): 59-70.

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