Research Article
Volume 6 Issue 7 - 2021
Metagenomic Profiling of Bovine Milk from Mastitis Infected Udder of the Cows before and after Treatment with Ethno-Veterinary Practice (EVP)
Santhosha Hegde1, Pavithra Narendran1, Malali Gowda2 and M N Balakrishnan Nair2*
1University of Trans-Disciplinary Health Sciences and Technology (TDU), Yelahanka, Bengaluru, India
2DNA Life, Yelahanka, Bengaluru, India
*Corresponding Author: M N Balakrishnan Nair, Professor, Centre for Ethno-Veterinary Science and Practice, University of Trans-Disciplinary Health Sciences and Technology (TDU), Yelahanka, Bengaluru, India.
Received: May 17,2021; Published: June 25, 2021




Abstract

This study presents the changes in the microbiome of milk from the cows with clinical mastitis before and after treatment with Ethno-veterinary herbal formulations. The mastitis was confirmed with CMT (California Mastitis Test). Besides presence of several species of bacteria, the mastitis causing bacteria were abundant before treatment. After 6 days of treatment with herbal formulations, the average abundance of Staphylococcus was reduced from 40.59% to 2.03% (20 times), Streptococcus from 25.8% to 2.06% (12.52 times), Pseudomonas, Pseudomonaceae family 20.28% to 1.9% (10.67 times), Klebsiella from 8.4% to 0.26% (32.31 times) and Enterobacteriaceae family from 24% to 1.69% (14.37 times) indicating the cure of mastitis.

 

Keywords: Mastitis; Metagenomic DNA; Ethno-Veterinary Practice

References

  1. Seegers Henri., et al. “Production effects related to mastitis and mastitis economics in dairy cattle herds”. Veterinary Research5 (2003): 475-491.
  2. Shome BR., et al. “Multiplex PCR assay for species identification of bovine mastitis pathogens”. Journal of Applied Microbiology6 (2011): 1349-1356.
  3. Erika Margarita Carrillocasas and Rosa Elena Miranda Morales. “Bovine Mastitis Pathogens: Prevalence and Effects on somatic cells count”. Open access reviewed Chapter in Milk Production - An Up-to-Date Overview of Animal Nutrition, Management and Health (2012).
  4. Wellenberg GJ., et al. “Viral infections and bovine mastitis: a review”. Veterinary Microbiology1 (2002): 27-45.
  5. Tarazona-Manrique LE., et al. “Bacterial and fungal infections etiology causing mastitis in dairy cows in the highlands of Boyacá (Colombia). [Etiologia bacteriana y micótica infecciosa causante de mastitis en vacas lecheras en el altiplano boyacense (Colombia)]”. La Revista de Medicina Veterinaria de la Facultad3 (2019): 208-218.
  6. Ranganathan V. “Ethno-veterinary practices for combating antimicrobial resistance”. International Journal of Science, Environment and Technology 1 (2017): 840-844.
  7. Nair MNB., et al. “Ethno-veterinary Formulation for Treatment of Bovine Mastitis, RRJVS/”. Journal of Veterinary Science S1 (2017): 25-29.
  8. Punniamurthy N., et al. “In-Vitro Antimicrobial Activity of Ethno-veterinary Herbal Preparation for Mastitis”. Journal of Dairy and Veterinary Sciences 2 (2017a): 555607.
  9. Punniamurthy N., et al. “Analysis of the mechanism of action by molecular docking studies of one ethno-veterinary herbal preparation used in bovine mastitis”. International Journal of Applied Nonlinear Science 5 (2017): 23-30.
  10. Kumar SK., et al. “Prevention of mastitis in cattle during dry period using herbal formulation”. RRJVS 1 (2018): 2581-3897.
  11. Katholm J., et al. “Evaluation of new qPCR test to identify the organisms causing high total bacterial count in bulk tank milk”. Journal of Integrative Agriculture6 (2018): 1241-1245.
  12. Katholm J., et al. “Quality of bulk tank milk samples from Danish dairy herds based on real-time polymerase chain reaction identification of mastitis pathogens”. Journal of Dairy Science 95 (2012): 5702-5708.
  13. Holmoy IH., et al. “Latent class analysis of real time qPCR and bacteriological culturing for the diagnosis of Streptococcus agalactiae in cow composite milk samples”. Preventive Veterinary Medicine 154 (2018): 119-123.
  14. Kuang Y., et al. “Characterization of Bacterial Population of Raw Milk from Bovine Mastitis by Culture-Independent PCR-DGGE Method”. Biochemical Engineering Journal 45 (2009): 76-81.
  15. Kennedy R., et al. “The microbiome associated with equine periodontitis and oral health”. Veterinary Research 47 (2016): 49.
  16. Andrews S. “FastQC: A Quality Control Tool for High Throughput Sequence Data (2010).
  17. Caporaso JG., et al. “QIIME allows analysis of high-throughput community sequencing data”. Nature Methods 7 (2010): 335-336.
  18. Aronesty E. “ea-utils: "Command-line tools for processing biological sequencing data". (2011)
  19. Edgar RC. “Search and clustering orders of magnitude faster than BLAST”. Bioinformatics 26 (2010): 2460-2461.
  20. DeSantis TZ., et al. “Green genes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB”. Applied and Environmental Microbiology 72 (2006): 5069-5072.
  21. Taponen S., et al. “Bovine milk microbiome: a more complex issue than expected”. Veterinary Research 50 (2019): 44.
  22. Bradley AJ. “Bovine mastitis: an evolving disease”. The Veterinary Journal 2 (2002): 116-128.
  23. Maoda Pang., et al. “Insights into the Bovine Milk Microbiota in Dairy Farms with Different Incidence Rates of Subclinical Mastitis”. Frontiers in Microbiology (2018).
Citation: M N Balakrishnan Nair., et al. “Metagenomic Profiling of Bovine Milk from Mastitis Infected Udder of the Cows before and after Treatment with Ethno-Veterinary Practice (EVP)”. EC Veterinary Science 6.7 (2021): 71-80.

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