For the last ten years, I have made major contributions to the field of ion channel biophysics and pharmacology in relation to neuronal and cardiovascular physiology and pathology. My background was in vascular physiology. I have explored the changes which occur in myometrium vasculature in late pregnancy (Prof George Osol laboratory, University of Vermont, USA, 2004-2005). I studied myo-endothelial interactions in systemic resistance vasculature (Dr Sergey Smirnov laboratory, University of Bath, 2006-2007). I investigated the effects of the gasotransmitters (NO, CO and H2S) and assessed their receptor sites on BKCa channels in recombinant systems and in the carotid body (Prof Paul J Kemp laboratory, Cardiff University, 2007-2009). I explored stoichiometry and kinetics of PI(4,5)P2 interaction with human Kv7.2/7.3 (M) channels and discovered ability of non-inositol phospholipids (NIPs) to stimulate opening of M-channel (Profs David A Brown (FRS) and Alasdair J Gibb laboratory, University College London, 2009-2012). I researched biophysical and physiological aspects of neurodegeneration in Huntington's Disease (Profs Nick D Allen and Paul J Kemp laboratory, Cardiff University, 2012-2016). I have an emerging reputation in the stem cell studies and developmental biology. In my studies I have particular attention to the mechanisms of neuronal and odontoblast differentiation in induced human pluripotent stem cell (hiPSC)-derived neurons and human telomerase reverse transcriptase dental pulp (hTERT DP) cells. After the new recent employment I have established a new independent neuroscience laboratory at Newcastle University School of Dental Sciences and at the current stage I am working towards developing new adequate cell models, which could be alternative to the primary culture cell models harvested from the animals with consequent aspirations to apply these models for the research of dental and orofacial post persistent pain management and exploring the cross-links with neurodegeneration, inflammation and aging.
I am interested in physiological aspects of neuronal differentiation, maturation, degeneration and pain. The aim of my research is to provide rational basis for the new therapeutic approaches for functional rescue of impaired neurons at persistent orofacial pain, neurodegeneration, inflammation and aging. In my research I concentrate on the mechanisms of prevention and/or relieve from pathological long-term conditions and prolonging a period of healthy and active living.